Fig. 2
Expression of genes related to inflammation and redox responses after combined exposure to DEPB0 and α-quartz. HBEC3-KT cells were exposed to 25 μg/mL (2.6 μg/cm2) DEPB0 and 50–100 μg/mL (5.3–10.5 μg/cm2) α-quartz for 2 h (i), 6 h (ii) and 12 h (iii), alone or in combination. The expression of CXCL8 (A), IL-1β (B), IL-1α (C), COX-2 (D) and HO-1 (E) was determined using qPCR. Results are expressed as fold change compared to unexposed cells and presented as boxplots of 4 independent experiments (Box: 25th-75th percentile, whiskers: minimum and maximum values, line: median, +: mean). Statistically significant differences were determined using two-way repeated measures ANOVA followed by Dunnett’s and Å Ãdák's post-tests and were calculated from the normalized CT values. *Statistically significant difference from the respective control at 0 μg/mL α-quartz. #Statistically significant difference between exposure groups