Fig. 5
SiNPs caused lipid peroxides accumulation in cardiomyocytes. A The cells were subjected to C11-BODIPY581/591, and imaged (a), correspondingly semi-quantified to calculate oxidized/reduced ratio (b). Scale bar, 50 µm. B The fluorescent intensity of cells labeled by C11-BODIPY581/591 was also measured using flow cytometry (a), and oxidized/reduced ratio was calculated for lipid ROS quantification (b). C GPX4 measurement by immunofluorescence. Scale bar, 25 or 50 µm. D GSH contents. E MDA contents. F Western blot assay. Data were expressed as mean ± SD. *p < 0.05 vs control