Fig. 5
From: Cellular response of keratinocytes to the entry and accumulation of nanoplastic particles
Effects of NPs on co-cultured dermal fibroblasts. The NPs released by the epidermis through the pores on the cell culture inserts were taken up by dermal fibroblasts (a) at 4 h (b) and 24 h (c) after epidermal treatment. The pro-collagen Ia levels in fibroblasts at 4 h (d) and 16 h (e) were estimated by immunofluorescence. The α-SMA levels in fibroblast cells were also measured after 4 h (g) and 24 h (h) NP treatment on co-cultured 3D keratinocyte cultures. The quantification of α-SMA levels was achieved by immunofluorescence as well (i). Scale bar: 50 μm