Fig. 11
From: Pro-inflammatory effects of inhaled Great Salt Lake dust particles
“Humanization” of mouse TRPV1 increased BAL neutrophils following GSLD PM2.5 inhalation. (a) Comparison of the amino acid sequence of the pore-loop region (residues 600/601–645) of human (h) and mouse (m) TRPV1. (b) Calcium flux data using HEK-293 cells transiently transfected with expression plasmids harboring human and mouse TRPV1, with and without mutations of residues 605/606 to the corresponding human and mouse residues. Nonivamide (20 mM; positive control, white bars), CFA (180 µg/cm2; light grey bars), and GSLD qPM2.5 (180 µg/cm2; dark grey bars) were used as agonists. Data are the mean and standard deviation. **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-way ANOVA and a Dunnett post-test. (c) comparison of BAL neutrophils in wild-type C57Bl/6 and Trpv1N606D “humanized” mice (3x exposure paradigm). Data are the mean and standard deviation. p < 0.05, **p < 0.01, and ***p < 0.001 using one-way ANOVA with Bonferroni post-test