Fig. 6

Redox proteomic analysis. Proteins found in each treatment group were filtered by p value/fold change and visualized via volcano plot (A) to demonstrate protein tendency to be oxidized (decreased fold change) or reduced (increased fold change) relative to control samples. The most significantly altered proteins and specific cysteine sites (B) consisted primarily of oxidized proteins associated with inflammatory signaling and ubiquitination. The subcellular location of significantly affected proteins (C) indicated uneven distribution across compartments, particularly the mitochondria. Redox proteomic data was normalized to general proteomic data at the protein level to account for changes in abundance unrelated to redox. Data was analyzed with an ANOVA and Tukey Honestly Significant Difference (HSD) test with a p value threshold of 0.05 to determine significance for subsequent analysis (N = 3)