Fig. 2
Effect of carbon particles on apoptosis and ROS generation in human neutrophils from responders and non-responders. Human neutrophils (2*106 cells/ml) from responder and non-responder donors were exposed to 33 µg/ml carbon nanoparticles (CNP) or carbon non-nanoparticles (CP). Controls were freshly isolated untreated neutrophils (0 h) or solvent controls (sham 1 h or 18 h). a, c apoptosis (% hypodiploidy) at 18 h after exposure. b, d DCF fluorescence determined at 1 h after exposure. Data are presented as mean ± SEM; a n = 123 (responder), n = 31 (non-responder); b n = 75 (responder), n = 21 (non-responder); c n = 20 (responder), n = 5 (non-responder), d n = 8 (responder), n = 3 (non-responder), *p ≤ 0.05