Fig. 6
Characteristics of Interstitial Macrophages. A–D: Triple staining for the myeloid lineage marker PU.1 (green in the nucleus), the macrophage marker CD68 (brown in the cytoplasm), and the AT1 differentiation marker Pdpn (red in the cell membrane) in the subpleural interstitium (A, bar: 50 μm), perivascular interstitium (B, bar: 25 μm), BALT (C, bar: 200 μm), and broncho-vascular bundle (D, bar: 100 μm) of a rat lung exposed to 8 mg/m3 TiO2 NPs. E: Masson Trichrome staining of a dust macule. F: Polarized image in E. Bar: 50 μm in E–F