Fig. 3

Differentially expressed genes in rat AM following overload exposure to P25 or Printex 90 particles. A-D. Representation of the 20 genes significantly differentially expressed in the AM in both rat experiments following overload of P25 and Printex 90 particles. A. Heatmap representing the logFC between overload (O) and control (CTL) or overload and non-overload (NO) conditions in rat (first and second HTS experiment) AM exposed to P25 or Printex 90 particles. The 20 genes were modulated in the same direction comparing the conditions, experiments and particles. B-C. Correlation tables representing the Pearson correlation coefficient (B) or the intraclass correlation coefficient (C) reflecting the very strong positive correlation or concordance of the logFC (overload vs. non-overload conditions) of the 20 genes between both rat HTS experiments and both tested particles. Stars indicate significant correlation: ***p < 0.001. D. Barplot representing the logFC between overload and non-overload conditions of the 20 genes comparing rat HTS analyses (first and second HTS experiment) and RT-qPCR analyses. RT-qPCR analyses were performed on 5 rat biological replicates. All genes, except Cadm3 and RT1-Db1 (in red), were similarly modulated comparing HTS and RT-qPCR analyses, after P25 and Printex 90 overload. E-G. Representation of the 18 genes (20 genes minus Cadm3 and RT1-Db1) confirmed to be differentially expressed in rat AM under P25 and Printex 90 overload. E. Correlation table presenting the Pearson correlation coefficient or the intraclass correlation coefficient reflecting the strong positive correlation or concordance of the logFC (overload vs. non-overload conditions) of the 18 genes between rat HTS experiments and RT-qPCR analyses. Stars indicate significant correlation: ***p < 0.001. F. Scatterplots comparing logFC values (overload vs. non-overload) between rat and human AM exposed to P25 or Printex 90 particles. Each dot represents one of the 18 genes. The small dots represent the logFC of each HTS experiment in rat AM while the big dots represent the mean of logFC of the two rat experiments. Each regression line indicates the linear relationship between the logFC of rat and human AM. The correlation degree between rat and human logFC was calculated by Pearson correlation coefficient R and the concordance of the values was calculated by the intraclass correlation coefficient ICC. A p-value < 0.05 indicates a significant correlation. G. Barplot representing the logFC (overload vs. non-overload conditions) of the 18 genes in rat (first and second HTS experiment) and human AM exposed to P25 or Printex 90 particles. Gene names are from reference genome rattus norvegicus